Cell Cytotoxicity Assay Kit (Suspended Target Cells)

 Cell LDH Cytotoxicity Assay Kit (Suspended Target Cells)

Cell Cytotoxicity is a critical quality attribute (CQA) for the biological activity of immune cell therapy products (e.g., NK cells, CAR-NK cells, Tm cells, CAR-T or TCR-T cells, etc.), and it is an important element of product quality research and quality control. Compared with cell cytotoxicity methods such as lactate dehydrogenase (LDH) assay, 51Cr release assay, luciferase reporter gene assay, RTCA assay, the detection of CFSE- and 7-AAD-labelled cell killing by flow cytometry is safer, more convenient, faster and more stable.

 

Features of Cell Pierce LDH Cytotoxicity Assay Kit (Suspended Target Cells)

01

Dual-Label Precision: CFSE labels target cells; 7-AAD identifies lysed cells.

 

02

High Throughput: Compatible with 96-well plates.

 

03

Safety: Eliminates radioactive isotopes (vs. 51Cr).

 

Specification of Car T Cell Cytotoxicity Assay Kit (Suspended Target Cells)

 

Application of Cell Cytotoxicity Assay Kit (Suspended Target Cells)

It is suitable for the evaluation of the killing function of immune cell products such as NK cells, CAR-NK cells, CAR-T cells, TCR-T cells, Tm cells, etc. on different tumour cell lines (suspended target cells).

 

CMC and quality research phase: In the early development phase of cell therapy kits, the cytotoxic effect is evaluated and the process is optimised.

 

Release testing, stability and comparability studies phase: During the mid-term process lock phase of cell therapy products, a cell killing method for the product is established, and release testing and stability testing of the cell product are performed.

 

Performance Index of Cell Cytotoxicity Assay Kit (Suspended Target Cells)

 

Data of Cell Cytotoxicity Assay Kits (Suspended Target Cells)

FACS analysis of the killing rate of NK cells against CFSE-labeled K562 target cells:

 

Data of Cell Cytotoxicity Assay Kit

 

Cell Cytotoxicity curves of NK cells against CFSE-labelled K562 target cells.

 

If you want to know more kinds of nk cell killing assay kits, please visit our website.

 


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