CD19 CAR-T Cell Preparation Kit

 CD19 CAR T cell preparation kit is composed of three parts: T cell activation and expansion unit, T cell purification unit and CD19 CAR gene transduction unit. The T cell activation and expansion unit consists of T cell basal medium and T cell activation reagent, which provides nutrients for T cell culture, and the T cell activation reagent provides T cell activation signals for T cell activation; the T cell purification unit consists of T cell sorting beads and columns, which is capable of obtaining high purity T cells from single blood collection or PBMC; The CD19 CAR gene transduction unit contains CD19 CAR T spot lentivirus and T cell transduction enhancer (Viral E-hancer B), which enables efficient integration of CD19 CAR sequence. This kit is easy to operate, and prepares CD19 Car T cells with high expansion multiplicity and cell viability, CAR gene transduction efficiency and T cell purity.

 

Application of CD19 CAR T Cell Preparation Kit

Applicable to the preparation of CD19 CAR-T cells of peripheral blood and cord blood origin.

 

Process development stage: shorten the time and cost of early process development of cell therapy products, and better help customers advance the development of CD19 CAR T cell therapy productsd.

 

Data of CD19 CAR T Cell Preparation Kit

Purity of T cells before and after sorting:

 

Cell viability and expansion:

 

T cell transduction efficiency:

 

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