Inorganic Pyrophosphatase ELISA Detection Kit

 This kit uses double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) method. Add PPase standard and test samples to the microtiter plate precoated with anti-PPase antibody, then add diluted biotin-labeled PPase detection antibody, finally add streptavidin-HRP to form the antibody + antigen + antibody-Biotin + SA-HRP complex, wash the plate and add TMB chromogenic solution for color development. TMB is converted from colorless to blue under the catalysis of HRP enzyme and finally to yellow under the action of stop solution. The shade of yellow is positively correlated with the amount of PPase detected in the sample.

 

Features of inorganic pyrophosphatase ELISA Detection Kit

01

Biotin-SA-HRP amplification boosts signal-to-noise vs. conventional ELISAs

 

02

Broad 64x dynamic range (0.25–16 ng/mL) – no dilutions needed

 

03

21 CFR Part 11-ready data templates for audits

 

General Workflow

Sample/standard preparation, sample loading, incubation and plate washing

Preparation of detection antibodies and plate washing

Preparation of enzyme-conjugated antibodies and plate washing

Color development and reaction termination

Reading

 

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